Microbiology Lab Identification Of Unknown Bacteria Quizlet

There are two genera of bacteria that can appear as a streptococcus arrangement that we will take up in the lab: the genus Streptococcus and the genus Enterococcu s. The bacteria supported and their results for all of the tests supported are kept in a matrix, or table. Bacteria rarely live alone but in communities with other bacteria. Lab Paper Unknown Bacteria Lab Report- Microbio Microbio Preview text Unknown Bacteria Lab Report Intro Clinical laboratories need be able to test and identify any dangers related to the bacteria. Harmless microbes surround us; living with us and within us. MICROBIOLOGY MODULE Methods of Isolation of Bacteria Microbiology 112 Notes properties (Gram positive: purple; Gram negative: pink/red). Mycobacterium smegmatis doesn't need so many copies of the genes because it doesn't require the high production of proteins when it is growing slow. Learn microbiology lab identification unknowns with free interactive flashcards. Microbiology Unknown Lab Report. The purpose of this laboratory exercise is to evaluate the student’s knowledge and capabilities in identifying micro organism. INTRODUCTION Observations of bacteria with conventional bright field microscopy yield relatively little useful information. Explain a rapid biochemical test for the identification of Staphylococcus aureus? Explain the principle of nitrate reduction test?. A microorganism, or microbe, is a microscopic organism, which may exist in its single-celled form or in a colony of cells. Students grow bacteria cultures on agar in petri dishes and do a plate count. MATERIALS AND METHODS An unknown labeled 125 was handed out by the lab instructor along with the instructions to use the procedures from previous labs to determine the identity. Price is per isolate. com Intense pink Light, uneven pink. The reason for identification of unknown bacteria was to help students recognize different bacteria through different biochemical tests and characteristics. 7 % soft agar in a Petri dish or flat. Todar's Online Textbook of Bacteriology presents information on Gram-positive aerobic endospore=forming bacteria, including members of the genus Bacillus. microbiology laboratory class for the identification of an unknown bacterium. These cells do not have membrane-bound organelles. "Diagnostic Tests for Identification of Bacteria") of your unknown using heavy inoculum from your BHI plate. In microbial ecology, the identification of microorganisms helps us characterize biodiversity. Instead, they are found in mixed populations with many other species. UNKNOWN LAB REPORT. Just like in clinical settings on Earth, it is important to identify unknown pathogens for diagnosis and treatment. Glencoe/McGraw-Hill. Clinical Microbiology. In this lab report a number of tests and results will be discussed. LAB REPORT Biochemical identification of unknown bacteria Unknown ID 001 Nerjes Alsalman 3/18/09 Biochemical identification of unknown bacteria RESULTS: Morphological characteristics: Nutrient Agar Slant: The tube in 37oC: The amount of growth was slight, white in color, and had effuse form of growth. If unable to collect 3 mL of urine, collect in sterile specimen container (37777) or yellow top tube (104184) and transport urine specimens to the Microbiology Laboratory or refrigerate within 30 minutes. Microbiology Unknown Project - 2014. Microbiology. com makes it easy to get the grade you want!. This means that it could be harmful to you. 03%) of carbon dioxide. Clinical microbiology focuses on all aspects of patient infections from testing samples to identifying bacterial, viral, fungal, and parasitic agents that are contributing to the infectious process, to determining the susceptibility of microorganisms to various antimicrobial agents, and reporting the results to healthcare providers. The organisms selected include types you have studied this semester and some new genera. The major test reaction to use in Staphylococcus identification is the coagulase test reaction, which divides the genus Staphylococcus into 2 groups—coagulase negative species and coagulase positive species. Cultural Characteristics 3. Microbiology Interview Questions and Answers. The conclusion drawn from these various tests was that unknown is Enterococcus faecalis. Biochemical Test for Identification of Bacteria Introduction Staining provides valuable information about bacterial morphology, Gram reaction, and presence of such structures as capsules and endospores. Identifying Enterobacter aerogenes from a Mixed Culture of Unknown Gram Positive and Gram Negative Bacteria Kevin Le November 13, 2013 PURPOSE The focus of this study is to be able to identify an unknown bacterium, specifically a gram negative one,. Lab 7 will demonstrate that different bacteria, because of their unique enzymes, are capable of different biochemical reactions. CNA agar is a type of blood agar. Use Gram staining to see if bacteria are Gram positive or Gram negative. 0 µm in diameter, usually seen in pairs with adjacent flattened sides (Figure 1 Left and Fig 2 below). Identification of Bacteria: Step # 1. The book aligns with the curriculum guidelines of the American. A colony is defined as a visible mass of microorganisms all originating from a single mother cell. I have developed software applications that will enable users to identify the organisms based on the results of their tests. The oxidase test is used to identify bacteria that produce cytochrome c oxidase, an enzyme of the bacterial electron transport chain. Each pair will receive one unknown organism to identify. The I dentification M atrix shows your assigned unknown with the results you have recorded for it in the. Morphology and. microbiology lab practical information part 1 - Duration: 11:54. Explore clinical microbiology techniques, such as differential staining, differential and selective media and biochemical assays, then use your new skills to. Study Microbiology lab exercise 46- Bacteria of the respiratory tract flashcards. Observing bacteria in a Petri dish. Lab 7 will demonstrate that different bacteria, because of their unique enzymes, are capable of different biochemical reactions. Explain a rapid biochemical test for the identification of Staphylococcus aureus? Explain the principle of nitrate reduction test?. These early systems included the nine-test Enterotube (Roche, Basel, Switzerland) and two-tube R-B systems for identifying members of Enterobacteriaceae. Asked in Microbiology, Bacteria, Microscopes Half Answer: There are both Gram positive and Gram negative stains that are used to identify different types of Bacteria. These taxa have been underreported, and the taxa were taxonomically confusing. UNKNOW BACTERIA LAB REPORT UNKNOWN 36 Introduction The purpose of this lab was to identify two unknown bacteria from a mixed culture. Dichotomous Key for identification of Bacteria. First examine the given sample undermicroscope in 10x, 40x and higher if available. In microbiology, I received an unknown bacteria of some sort that I am supposed to identify by Species and genus. In our lab, identification of unknown bacteria is divided into 3 projects; EU, OU and PCR-ID projects. These include P aeruginosa, P fluorescens, P putida, P cepacia, P stutzeri, P maltophilia, and P putrefaciens. Samples of blood, tissue, food, water and cosmetics are examined daily in laboratories throughout the world for the presence of contaminating microorganisms. Lab Report #3b. One use of a dichotomous key is to organize large amounts of information so identifications can be made more easily. This study was done by applying all the knowledge in the microbiology laboratory class for the identification of an unknown bacterium. main idea of this experiment was to correctly identify the unknown bacteria, #3. Unknown Microbiology Lab Report. BIO-243 Microbiology Unknown Bacteria Identification Introduction Microbiology is the study of microorganisms that may or may not be visible to the naked eye. The methods that have been learned thus far for identifying bacteria have been applied to this unknown. This study was done by applying all of the methods that have been instructed on thus far in microbiology laboratory class. If you have any questions about this stuff, feel free to email me at:. TSA is a typical medium used for cultivation of samples where many types of microorganisms might be expected. Each clue will offer possibility to solve the puzzle. Only two species, P mallei and P pseudomallei, produce. A broth tube labeled number 102 was received from the lab instructor with two unknown bacteria inside. Harmless microbes surround us; living with us and within us. Sterilization and disinfection. ) with full. The purpose of the unknown lab is to apply the systematic reasoning learned in class to identify the prokaryotic bacteria in two unknown samples. Newman, Lycoming College (srv2. Each test performed, provided us with some key information about the unknown organism in question and how the bacteria. Rapid Identification Methods. edu 1 IDENTIFICATION OF OTHER UNKNOWN BACTERIAL SPECIES: OU I. Each test performed, provided us with some key information about the unknown organism in question and how the bacteria. 5 m M MgCl 2. Grade: _____ Hunter Larson GUIDELINES 1. Access Google Sites with a free Google account (for personal use) or G Suite account (for business use). meningitidis, S. The first step that was done was taking a Trypticase Soy Agar (TSA) plate, and using the quadrant streak method as described in the lab manual, the unknown was streaked onto the plate. Therefore, viral specificity can be used sometimes as a tool for identifying unknown bacteria. Identification of unknown bacteria using dichotomous key-Part 1 Medical Lab Lady Gill 15,810 views. A colony is defined as a visible mass of microorganisms all originating from a single mother cell. Unknown Lab Report: Unknown "C" Discussion/Results. Use the Alphabetical Test Directory above to display listings of all frequently ordered tests performed within CLS as well as those performed at other medical laboratory testing facilities. This is the microbiology questions and answers section on "Identification of Bacteria" with explanation for various interview, competitive examination and entrance test. com Intense pink Light, uneven pink. Clinical Specimens From Intestinal Tract, Culturing A Fecal Sample, Identification An Unknown Enteric Organism, Microbiology Intestinal Tract. Gram stain: A method of differentiating bacterial species into two large groups (Gram-positive and Gram-negative). A broth tube labeled number 102 was received from the lab instructor with two unknown bacteria inside. The point of this study was to identify two unknown bacteria by employing all methods that had been used and learned about throughout the Microbiology Laboratory class. Identification of Unknown Bacteria. Other than that, being able to isolating and identify bacteria also is the basic knowledge and requirement for lab study, which requires high accuracy. Tubes must be filled to 3 mL to prevent inhibition of bacterial growth. These microorganisms range from deadly to vital for life and health to continue. Aug 10, 2016 - Need Some Help On Determining A Bacteria - d2jsp Topic. microbiology lab practical information part 1 - Duration: 11:54. positive and gram-negative bacteria whereas narrow-spectrum antibiotics may only work against a small class of bacteria, e. The identification of the unknown bacteria in this study was completed by using methods learned in the microbiology lab class for BIO203. Second lab in a series on identifying unknown bacteria. Jul 12, 2014 - This Pin was discovered by Pam Smith. Lab_exercise_3b_id_of_unknown. Instead, they are found in mixed populations with many other species. Spring 2013 INTRODUCTION. General Microbiology Laboratory Manual - Minot. The lab instructor gave out an unknown labeled 124, which contained a gram positive bacterium and a gram negative bacterium. A total of 5,137 enterobacterial clinical isolates were examined with three series of biochemical tests. The following tests were performed on the gram-­‐positive bacteria: 1. The point of this study was to identify two unknown bacteria by employing all methods that had been used and learned about throughout the Microbiology Laboratory class. The procedures taken to figure out, and isolate the two separate bacteria were taught thus far in Microbiology lab class. April 29 th, 2014. The bacteria supported and their results for all of the tests supported are kept in a matrix, or table. Experiment for identification of unknown bacteria! Principle: Identification of unknown bacteria is one of the major responsibilities of the microbiologists. coli cells to become cit+. In microbiology laboratory class many techniques were studied to learn how a microorganism will react in certain conditions. , organisms that require a lot of growth factors, known or unknown). This Concept Map, created with IHMC CmapTools, has information related to: Kingdom Protista, are multi-cellular, toxins kill most fish, habitats such as freshwater, produce silicon dioxide, diatoms live in habitats, thickener in instant pudding, diatomaceous earth which is used as filter of liquids, agar used for foods, cell walls made of cellulose, Freshwater typically on decaying vegetation. The identification is required so as to cure the. Because the primary purpose of this manual is to aid in the identification of N. 13 Preliminary tests used to identify gram-positive aerobes include the presence or absence of hemolysis on a blood agar plate (Figure 3-7), a positive or negative catalase test, and the production of coagulase. The differences in DNA sequence can be used to identify organisms. Each test performed, provided us with some key information about the unknown organism in question and how the bacteria. This particular study was conducted by using all of the methods learned this past semester in order to understand and identify unknown bacterium. Answer to can someone help me with a sample microbiology lab report on an unknown bacteria?? Skip Navigation Can Someone Help Me With A Sample Microbiology Lab Report On An Unknown Bacteria?? This problem has been solved! Aim: Identification of bacteria, sample from urinary tract. Isolation and Identification of Gram (+) Organism #13 - Bacillus subtilis 4. Materials and Methods. It can help form proper antibiotics and even new antibiotics. Identifying Enterobacter aerogenes from a Mixed Culture of Unknown Gram Positive and Gram Negative Bacteria Kevin Le November 13, 2013 PURPOSE The focus of this study is to be able to identify an unknown bacterium, specifically a gram negative one,. Identification Of Unknown Bacteria Microbiology Laboratory Exercise (Part II) Lab Report #3b – ID of Unknown. Microbiology BIOL 275 Dr. In microbiology, differential staining techniques are used more often than simple stains as a means of gathering information about bacteria. The methods that have been learned thus far for identifying bacteria have been applied to this unknown. Through out the semester in the laboratory, we are able to encounter some of the few microorganisms that we as humans. Most of the currently known species of bacteria have been identified using traditional microbiological techniques such as the gram stain reaction, morphology, and metabolic reactions. The bacteria in and on our bodies makes up about 10% of our dry body weight. Unknown Lab Report: Unknown "C" Discussion/Results. Media used for identification of bacteria by testing various biochemical reactions are sorted alphabetically and by type of test. Bacteria rarely live alone but in communities with other bacteria. Morphological appearance of bacterial colonies was often sufficient for their identification in the laboratory. Study Microbiology lab exercise 46- Bacteria of the respiratory tract flashcards. In order to isolate the two unknown bacteria from the single test tube, an isolation streak plate needed to be performed. MATERIALS AND METHODS. Characteristics of bacterial colony growth are described in terms of shape, appearance, and color. Coli, Klebsiella Pneumoniae, Proteus Vulgaris Pseudomonas Aeruginosa, Salmonella Typhimurium and the gram + are: enterococcus faecalis, micrococcus luteus, staph. Quickly memorize the terms, phrases and much more. Unknown Bacteria Lab Report Introduction The purpose to this lab was to identify an unknown bacteria from a mixed culture provided to us by our instructor. There weren't as many study guides for this particular class because I had several other classes that semester which required a lot more attention (*cough* Orgo II *cough*). Standard Microbiological Practices 1. and observe the. The critical nature of the microbiology laboratory in infectious disease diagnosis calls for a close, positive working relationship between the physician/advanced practice provider and the microbiologists who provide enormous value to the healthcare team. Identifying bacteria can lead to many new discoveries such as new species or the evolution of a known species. Unknown Number 109. Aid in proficiency in submitting specimens for the identification of infectious disease in your future patients. (1) lab manual. Microbiology Unknown Lab Report Essay 2396 Words | 10 Pages. NAME OF EXERCISE. The lab-report genre does have several sections you’ll need to meet in your report, which include: a Purpose Statement, Methods, Results, a Discussion or Conclusion, and References. Due to its immense size, about 60 micrometers cubed, B. The point of this study was to identify two unknown bacteria by employing all methods that had been used and learned about throughout the Microbiology Laboratory class. Bacteria are a causative agents for many diseases in humans and domestic animals that and can be a cause of significant health impact. Only two species, P mallei and P pseudomallei, produce. ), carbon source utilization (Microlog; Biolog, Inc. Urine isolates are tested by an automated system. - 48 cards; Microbiology. Your ability to make aseptic transfers, perform the gram stain,. In this paper I will discuss the processes of how I came to find my two unknown bacteria. A comprehensive database of more than 11 microbiology lab quizzes online, test your knowledge with microbiology lab quiz questions. On August 24, 2015 May 13, 2016 By David Fankhauser In Lab Protocols, Lecture Notes Leave a comment Microbiology 2031 Lecture Notes in PDF Format 01_History_Microbiology Aug15. The purpose of our study is to determine the identity of two unknown bacterium, #2 and #18, which has been presented and discussed by the professor during lab this semester. The organisms selected include types you have studied this semester and some new genera. Microbiology. If unable to collect 3 mL of urine, collect in sterile specimen container (37777) or yellow top tube (104184) and transport urine specimens to the Microbiology Laboratory or refrigerate within 30 minutes. Directory of Tests List of microbiology tests in lab. Unknown test tube culture #31 was selected. Because of the large size of amylose and amylopectin molecules, these organisms can not pass through the bacterial cell wall. This test is used to identify bacteria that can hydrolyze starch (amylose and amylopectin) using the enzymes a-amylase and oligo-1,6-glucosidase. Conventional identification methods based on biochemical tests are often lengthy and can produce inconclusive results. id of unknown bacteria laboratory documents This laboratory topic has four associated Word documents. Expensive equipment and materials are not usually necessary for recovery of common aerobic or facultatively anaerobic bacterial pathogens, such as Staphylococcus spp, Streptococcus spp, and coliforms. ) with full confidence. It is not conserved, its sequence is not the same in all bacteria. Your ability to make aseptic transfers, perform the gram stain, identify. of bacterial cells. Students Name: Hunter Larson Unknown ID Number: 3 Genus & Species: Proteus vulgaris. microbiology lab practical information part 1 - Duration: 11:54. You have reached a page from The Cleveland Clinic Disease Management Project that is no longer available on this web site. Escheria coli) I have to pick two tests to narrow down what I have. Escherichia coli) which has two copies of the rRNA genes. Coli, was overtaken by mold! Not necessarily happy it happened but it looks cool & the teacher’s never seen it happen!. Skip to the end of the lab activity where it says "Prepared slides of typical bacteria" and view the prepared slides of bacterial shapes available in the laboratory. Self Evaluation. IDENTIFICATION OF UNKNOWN BACTERIA. Many times you have already performed or are presenting performing these diagnostic tests. UNKNOW BACTERIA LAB REPORT UNKNOWN 36 Introduction The purpose of this lab was to identify two unknown bacteria from a mixed culture. It need to identify on Molecular basis. The procedures that were followed throughout this identification can be found in the laboratory manual by McDonald Et. In this simulation, you will learn to identify unknown bacteria. TSA is tryptic soy agar. This is the microbiology questions and answers section on "Identification of Bacteria" with explanation for various interview, competitive examination and entrance test. It produces benefits for many aspects of the research of microorganisms and helps physicians correctly treat patients. Material &Method An unknown tube labeled number 20 was. Litmus milk decolorization test: To help identify Enterococcus and some Clostridia which have ability to metabolize litmus milk. Microbes in Water: Membrane Filter Technique. Aug 19, 2017 - This Pin was discovered by Carmen. The identification of the unknown culture was accomplished by identifying the bacteria based on its specific metabolic characteristics and morphology. The test tube contained a mixed culture of two unknown bacteria, one a gram positive and one a gram negative. It is not conserved, its sequence is not the same in all bacteria. Sarah Ruble Medical. Often used to differentiate species from the genera Clostridium and Bacillus. Phenotypic characteristics used as a means of identification for fungi rely on microscopic morphology for accurate and correct identification2. It can help form proper antibiotics and even new antibiotics. Bacteria rarely live alone but in communities with other bacteria. The identification of these unknown cultures was accomplished by separating and differentiating possible bacteria based on specific biochemical characteristics. positive and gram-negative bacteria whereas narrow-spectrum antibiotics may only work against a small class of bacteria, e. The test media that you will run for identification depends on which category your organism falls in. Typing of Bacteria: Bacteriophage Sensitivity 6. edu is a platform for academics to share research papers. Most of the currently known species of bacteria have been identified using traditional microbiological techniques such as the gram stain reaction, morphology, and metabolic reactions. Bacteriophages can be grown in the presence of a dense layer of bacteria (also called a bacterial lawn) grown in a 0. In this lab, students identify an unknown bacteria using a biochemical method and a molecular method. ADVERTISEMENTS: The following points highlight the seven steps for identification of bacteria isolated from a specimen. All work will be done by individuals or teams. The Enterotube™ II is an example of a rapid, multi test system used in identification of unknown oxidase- negative, gram- negative, rod shaped bacteria of the family Enterobacteriaceae. The Lab Exercise Instructions Part I provide a thorough explanation of streak plating and how to prepare bacterial smears for differential staining. In lab studies, Anthony demonstrated that the virus can infect human cells but less efficiently than Ebola Zaire. 10/8 at 11:59 PM. Left: Neisseria gonorrhoeae Gram stain of pure culture; Right: Neisseria gonorrhoeae Gram stain of a pustular exudate. UNKNOW BACTERIA LAB REPORT UNKNOWN 36 Introduction The purpose of this lab was to identify two unknown bacteria from a mixed culture. Methods The tests performed throughout the process of identifying unknown samples 16 A and 16 B were followed exactly as specified in the text Microbiology: Laboratory Theory & Application (Leboffe. Genome Terminology. What bacteria is a basic identification sufficient i. Please read the procedures before coming to lab so you have an idea of what will be done that day. Study Laboratory Experiments in Microbiology-Exercise 7 flashcards. lab period 1) Inoculate 2 Different TSA (Agar) Slants Using Single Line to Get a Growth Pattern 2) Inoculate 3 Different TSB (Broth) Tubes Using 3 Loop-Fulls of Your Unknown, to Determine the Growth Pattern in Broth and to Determine the Optimal Growth Temperature. Biology Online - Information in the life sciences in the form of tutorials, references, an online dictionary of biology terms and a biology forum. Use arrow keys for next and previous. Title: Dichotomous Key for Identifying Unknown Bacteria Author: Tami Keywords: microbiology dichotomous key, dichotomous key identify bacterial unknown, free microbiology dichotomous key Created Date:. The Human Skin Microbiome Project is an application of the principles and practices of classic microbiological investigation. Identification and characterisation of microorganisms is a key part of the management of food safety and quality, tracing contaminants and troubleshooting problems such as spoilage. Principle: The identification of bacteria is a careful and systematic process that uses many different techniques to narrow down the types of bacteria that are present in an unknown bacterial culture. Known bacteriophages are used to identify unknown bacteria by observing whether or not the bacteria are lysed. Dec 14, 2014 - gram negative bacilli flowchart | Gram Negative Rod Bacteria Bacillus is a rod shaped. Identification of unknown bacteria using dichotomous key-Part 1 Medical Lab Lady Gill 15,810 views. Phenotypic characteristics used as a means of identification for fungi rely on microscopic morphology for accurate and correct identification2. This test is used to identify bacteria that can hydrolyze starch (amylose and amylopectin) using the enzymes a-amylase and oligo-1,6-glucosidase. Rebecca Lashley. The techniques used were applied to identify an unknown bacterium. Example of a Microbiology Unknown Lab Report by Taylor Autry Introduction. Microbiology Lab Cyanobacteria, Protozoans, and Algae Prokaryotic and Eukaryotic Cells As you know, the building blocks of life are cells. tuberculosis or other acid-fast bacteria are present, though samples from elsewhere in the body (such as urine) may contain other Mycobacterium species. There is an online GLOSSARY OF TERMS, linked to terms within the text of the lab exercises. All work will be done by individuals or teams. Introduction: There are microorganisms on every single thing in our world. BIO-243 Microbiology Unknown Bacteria Identification Introduction Microbiology is the study of microorganisms that may or may not be visible to the naked eye. Clinical microbiology laboratory plays several important roles in the management of bacterial infections. Being able to culture, isolate and identify a unknown bacteria is one of the corner stones in microbiology BIO 203, as well as the ability to use different techniques to distinguish and identify different bacteria. Grade: _____ Hunter Larson GUIDELINES 1. 0 µm in diameter, typically occurring in pairs and chains of varying length when grown in a liquid medium, and often occurring singly, in pairs, short chains, and clusters. For EU (identification of a bacterial species belonging to the Enterobacteriaceae family) and OU (identification of a non-Enterobacteriaceae bacterial species), we use the conventional microbiological diagnostic techniques to identify the species. d) All of the above. Microbes in Water: Multiple-Tube Technique. This shockingly minimal percentage lends credence to the idea that increasing humankind's knowledge of unknown bacteria could have great future benefits. edu 1 IDENTIFICATION OF OTHER UNKNOWN BACTERIAL SPECIES: OU I. All of the following tests were performed on this unknown: Procedure Purpose Reagents Results. Morphology and. The identification of the unknown culture was accomplished by identifying the bacteria based on its specific metabolic characteristics and morphology. Name:_____Lab Partner:_____. Staining not only makes bacteria more easily seen, but it allows their morphology (e. Aug 10, 2016 - Need Some Help On Determining A Bacteria - d2jsp Topic. Aid in proficiency in submitting specimens for the identification of infectious disease in your future patients. The results of the biochemical tests along with deductive reasoning and elimination led to the identification the unknown bacteria. I'll use the manner for my own document. In this activity you will observe the effects of antibiotics on bacteria that were cultured in a petri dish. The identification of the unknown culture was accomplished by identifying the bacteria based on its specific metabolic characteristics and morphology. Microbiology Laboratory Techniques Chapter Exam Take this practice test to check your existing knowledge of the course material. The reason for identification of unknown bacteria was to help students recognize different bacteria through different biochemical tests and characteristics. There are some basic differences between Bacteria, Archaea, and Eukaryotes in cell morphology and structure which aid in phenotypic classification and identification. Principle: The identification of bacteria is a careful and systematic process that uses many different techniques to narrow down the types of bacteria that are present in an unknown bacterial culture. Reference. Knowing how to identify bacteria or other unknown microorganisms that you have encountered can help to assess whether it poses a safety/spoilage concern or is. Hemolysis of blood cells can be very useful as an identification test. Lab Report #3b. 2016 Microbiology Laboratory Manual - Page 32 Microbiology Experiment #7 - Exoenzymes, Differential and Selective Media Every microorganism has different metabolic capabilities. microbiology laboratory class for the identification of an unknown bacterium. One way is to. id of unknown bacteria laboratory documents This laboratory topic has four associated Word documents. General Microbiology Lab Manual MB 303 Fall 2017 ©OSU Department of Microbiology, 2010 Instructors: Dr. Most pseudomonads known to cause disease in humans are associated with opportunistic infections. Learn vocabulary, terms, and more with flashcards, games, and other study tools. Microbiology Unknown Lab Report Essay 2396 Words | 10 Pages. The point of this study was to identify two unknown bacteria by employing all methods that had been used and learned about throughout the Microbiology Laboratory class. The identification of bacteria is a careful and systematic process that uses many different techniques to narrow down the types of bacteria that are present in an unknown bacterial culture. IDENTIFICATION OF UNKNOWN BACTERIA. MATERIALS AND METHODS. The procedures following the various different methods can be found in our lab manual. positive and gram-negative bacteria whereas narrow-spectrum antibiotics may only work against a small class of bacteria, e. There are several staining methods that are used routinely with bacteria. Here is a small number of study guides that I made for a Microbiology class that I took in fall 2011. , Newark, Del. Optional: If you like to know the motility of your species, you may stab a motility butt. al (1), unless noted within this lab report. MICROBIOLOGY AND THE ENVIRONMENT. Unknown C was determined to be a Gram- negative rod. Learning Objectives. Providencia stuarti. Coli and the results were given as such: 1: >80. The broth contains two pH buffers, urea, a very small amount of nutrients for the bacteria, and the pH indicator phenol red. Some bacterial genomes are comprised of multiple chromosomes and/or plasmids and many bacteria harbor multiple copies of their genome per cell. BIO 203-604 Introduction. Rebecca Lashley. In this lab, students identify an unknown bacteria using a biochemical method and a molecular method. Describe examples of biosynthesis products within a cell that can be detected to identify bacteria; Accurate identification of bacterial isolates is essential in a clinical microbiology laboratory because the results often inform decisions about treatment that directly affect patient outcomes. Mycobacterium smegmatis doesn't need so many copies of the genes because it doesn't require the high production of proteins when it is growing slow. For example, in food microbiology it is important to be able to accurately identify food spoilage contaminants. The lab exercises you cover while working on your unknown will be on your lab practical, and your lab practicals count for much more of your lab grade than your unknown. Each Q is worth 2 points unless otherwise noted. Unknown Number 115. We'll review your answers and create a Test Prep Plan for you based. and observe the. Best Answer 100% (4 ratings) You will draw upon many of your new skills to identify unknown bacteria. (3 pts) Section 2 Biochemical Tests. Lab #1 video clips Download Lab 1 ; Lab #2 Download Lab 2; First Unknown Dichotomous Keys for labs 2-4 (Printable Version. Linda Bruslind - Monday & Tuesday lab Lab Notebook Due Lab Final Ex. Lab Report #3b. UNKNOWN LAB REPORT. Describe role of microbiology in the eyes and skin. However, there are a few cocci shaped gram negative bacteria as well. A diverse range of biochemical reagents are known for the identification of certain metabolisms and to differentiate between bacteria. Tubes must be filled to 3 mL to prevent inhibition of bacterial growth. Lab Paper Unknown Bacteria Lab Report- Microbio Microbio Preview text Unknown Bacteria Lab Report Intro Clinical laboratories need be able to test and identify any dangers related to the bacteria. [ April 14, 2020 ] SARS-CoV-2 IgG/IgM Rapid Test for the diagnosis of COVID-19 Lab Diagnosis of Viral Disease [ April 4, 2020 ] Collection and Transport of Nasopharyngeal Swab Virology. The identification is required so as to cure the. Lansing, Harley P. Also, per the Honor Code, this work must be your own. Access to the laboratory is limited or restricted at the discretion of. The differences in DNA sequence can be used to identify organisms. Microbiology Laboratory: Test: Identification of Unknown Bacteria and Fungi by DNA Sequencing: Description: EMSL can identify your unknown, pure, fungal or bacterial isolates using PCR and DNA sequencing. The Gram stain is a differential stain commonly used in the microbiology laboratory that differentiates bacteria on the basis of their cell wall structure. Since this lab is about identifying bacterial unknowns, you may also have to look at previous lab exercises to interpret your observations, and to answer questions on. It is necessary that you read the chapter on diagnostic tests carefully before performing the actual inoculations. MICROBIOLOGY UNKNOWN LAB - SCIENTIST CINDY. Laboratory Methods in Microbiology is a laboratory manual based on the experience of the authors over several years in devising and organizing practical classes in microbiology to meet the requirements of students following courses in microbiology at the West of Scotland Agricultural College. The results of these tests on the suspected microorganism are then compared to known results for that organism to confirm its identification. Micro Lab 4: Bacterial Structure, Simple Stains, Negative Stains, Gram & Acid-Fast Stains - Duration: 28:08. Report on Identification of Mixed Culture Unknown Lab Exercise 33. The blood agar plate is a differential media that distinguishes between hemolytic and non-hemolytic bacteria. Microbiology. Rapid identification and susceptibility testing are still major challenges in recent medical microbiology, in particular in the era of multi-/pan-resistant microorganisms. Inoculate a trypticase soy 5% sheep blood agar plate by streaking a heavy inoculum onto one-fourth of the plate and streak the remaining portion for isolated colonies. 2012 - Unknown organism was streaked from a mixed broth onto both TSA and BHA plates in an attempt to isolate pure colonies for testing. Purpose: To identify an unknown bacterial specimen using basic laboratory technique and biochemical tests. Salmonella serotyping is a subtyping method of identification based on the identification of distinct cell wall, flagellar, and capsular antigens with known antiserum, as will be discussed in Lab 17. Directory of Tests List of microbiology tests in lab. Identification of Bacteria: Step # 1. "Diagnostic Tests for Identification of Bacteria") of your unknown using heavy inoculum from your BHI plate. aureus, staph epi. Identification of bacteria is necessary because. Most pseudomonads known to cause disease in humans are associated with opportunistic infections. The blood agar plate is a differential media that distinguishes between hemolytic and non-hemolytic bacteria. UNKNOW BACTERIA LAB REPORT UNKNOWN 36 Introduction The purpose of this lab was to identify two unknown bacteria from a mixed culture. Unknown C was determined to be a Gram- negative rod. laboratory and are supervised by a scientist with general training in microbiology or a related science. These early systems included the nine-test Enterotube (Roche, Basel, Switzerland) and two-tube R-B systems for identifying members of Enterobacteriaceae. Biochemical Reactions 4. Morphology and Staining 2. Lab_exercise_3b_id_of_unknown. THE RELATIONSHIP OF IDENTIFICATION TO BACTERIAL CLASSIFICATION AND NOMENCLATURE. The size of the zone of inhibition for a particular antibiotic is influenced by diffusibilty of the agent, size of the inoculum, type of medium, and resistance mechanisms of. Megan McBride. Coli and the results were given as such: 1: >80. Oxygen requirements of bacteria reflect the mechanism used by them, to satisfy their energy needs. If working in teams: each investigating student must turn in a report. Microbiology. al (1), unless otherwise noted. A major innovation in laboratory sciences occurred in the late 1960s, when manual miniaturized identification systems were first introduced into the clinical microbiology laboratory. However, the bacterial unknowns project may just be the ultimate assessment an instructor can implement in the microbiology laboratory. Biology Online - Information in the life sciences in the form of tutorials, references, an online dictionary of biology terms and a biology forum. Although most bacteria are produced with single microscopic cells, they are not as easy as previously believed. Micah Schenck. A Gram stain also helps to identify mixtures of bacteria, helps to determine the appropriate range of agar plates to be used for subsequent culture, and helps with the interpretation of culture results. Microbiology Laboratory: Test: Identification of Unknown Bacteria and Fungi by DNA Sequencing: Description: EMSL can identify your unknown, pure, fungal or bacterial isolates using PCR and DNA sequencing. Answer to can someone help me with a sample microbiology lab report on an unknown bacteria?? Skip Navigation Can Someone Help Me With A Sample Microbiology Lab Report On An Unknown Bacteria?? This problem has been solved! Aim: Identification of bacteria, sample from urinary tract. Draw a picture of the bacillus shaped bacteria. In addition to Bunsen burner and inoculating loop, different medias to culture and grow the unknown were assigned. Hence, it's very important to check for fungal species if the culture and sensitivity test comes back negative for bacteria. Some of these organisms have distinct qualities that set them apart from one another, such as the exposure to certain environments. The purpose of this laboratory exercise is to evaluate the student's knowledge and capabilities in identifying micro organism. This lab report is worth 100 points towards your final lab grade. Unknown labs are performed for many reasons ranging from figuring out the causative agent in a diseased patient, to knowing the correct microorganism to use when making certain foods and antibiotics. What is "16S rDNA," and how is it used to identify species of bacteria? Click to Enter the Lab. MATERIALS AND METHODS. When working in a microbiology lab, it is important to contain spills and discard materials properly. Soil Bacteria 83 Each student begins the procedure of isolating an unknown bacterium from soil during our first laboratory session. In most cases, identifying bacteria is done through a process of elimination to narrow down choices until you get to the right one. Often used to differentiate species from the genera Clostridium and Bacillus. The MLG contains methods for the sample preparation, isolation and identification of the major foodborne pathogenic microorganisms and their toxins, meat tissue species identification, and the. The micro lab website, your textbook, the web and assorted books available in lab will be the reference materials necessary for you to successfully complete the next several weeks of lab work. Each module demonstrates a different laboratory test, and the student is able to carry out and interpret the. al (1), unless otherwise noted. A broth tube labeled number 102 was received from the lab instructor with two unknown bacteria inside. Urease Test Urease broth is a differential medium that tests the ability of an organism to produce an exoenzyme, called urease, that hydrolyzes urea to ammonia and carbon dioxide. Just like in clinical settings on Earth, it is important to identify unknown pathogens for diagnosis and treatment. The methods that were previously studied and practiced in the Microbiology laboratory class were applied in order to identity two unknown bacterium. Even in modern times, preliminary identification of most cultivable bacteria is based on such morphological characters. There are several staining methods that are used routinely with bacteria. Infectious agents are discussed in relation to their morphology, biology, epidemiology and pathogenesis. biochemistry. :) Organized Microbiology Gram Stain Flow Chart Unknown Bacteria Flow Chart Flowchart To Identify Unknown Bacteria Microbiology Identification Flowchart. Samples of blood, tissue, food, water and cosmetics are examined daily in laboratories throughout the world for the presence of contaminating microorganisms. Although most bacteria are produced with single microscopic cells, they are not as easy as previously believed. The laboratory manual procedures by McDonald et. laboratory and are supervised by a scientist with general training in microbiology or a related science. tuberculosis or other acid-fast bacteria are present, though samples from elsewhere in the body (such as urine) may contain other Mycobacterium species. Jul 12, 2014 - This Pin was discovered by Pam Smith. Motility agar has a colorless dye (TTC or 2,3,5-Triphenyltetrazolium Chloride) that some bacteria have the ability to reduce. Rebecca Lashley. Discover (and save!) your own Pins on Pinterest. The reason for identification of unknown bacteria was to help students recognize different bacteria through different biochemical tests and characteristics. Eby Bassiri [email protected] My group’s EMB plate, which contained some E. The identification of the unknown bacteria in this study was completed by using methods learned in the microbiology lab class for BIO203. Second lab in a series on identifying unknown bacteria. The procedures following the various different methods can be found in our lab manual. 2012 - Unknown organism was streaked from a mixed broth onto both TSA and BHA plates in an attempt to isolate pure colonies for testing. On August 24, 2015 May 13, 2016 By David Fankhauser In Lab Protocols, Lecture Notes Leave a comment Microbiology 2031 Lecture Notes in PDF Format 01_History_Microbiology Aug15. Comparison to controls identified the bacteria as serratia marcescens. Understand the use of differential and selective media in the identification of bacteria and the enrichment of such cultures for organisms of interest. In this particular study an unknown was given by the professor. Phenol Red Broth is a general-purpose differential test medium typically used to differentiate gram negative enteric bacteria. Learn vocabulary, terms, and more with flashcards, games, and other study tools. For the Gram positive bacteria, I ended up with the my unknown #122 being Bacillius cereus. Bacillus megaterium is latin for the big beast because it is an extremely large bacteria, it is about 100 times as large as E. pneumoniae or H. Microbiology Unknown Lab Report. Some tests are used routinely for many groups of bacteria (oxidase, nitrate reduction, amino acid degrading enzymes, fermentation or utilization of carbohydrates); others are restricted to a single family, genus, or species (coagulase test for staphylococci, pyrrolidonyl. INTRODUCTION The identification of an unknown is beneficial in many ways, including the identification of bacteria in diseases, and in research. The use of stains that react chemically with cell material will. marcescens produces red colonies B. Expensive equipment and materials are not usually necessary for recovery of common aerobic or facultatively anaerobic bacterial pathogens, such as Staphylococcus spp, Streptococcus spp, and coliforms. edu/~newman), and Dr Dag Harmstad, University of Muenster, Germany (personal communication). The identification of the unknown bacteria in this study was completed by using methods learned in the microbiology lab class for BIO203. Microscope E1 was used to view unknown number 20 to determine whether the bacteria were Gram-positive or Gram-negative and cocci or bacillus. ALL of the following were performed on the unknown Phenol Red Broth, Methyl Red, Voges Proskauer, Citrate, Nitrate Reduction, Gelatinase, Urease, SIM. “ Our goal is to reach a deeper understanding of the pathophysiology of Hirschsprung-associated enterocolitis as well as the identification of novel treatments and prevention of the disease, ” said Gosain. laboratory and are supervised by a scientist with general training in microbiology or a related science. This study is the determination of the UNKNOWN bacterium that was given by Microbiology LAB Professor. Learn vocabulary, terms, and more with flashcards, games, and other study tools. Why Microbiology Identification of Bacteria? In this section you can learn and practice Microbiology Questions based on "Identification of Bacteria" and improve your skills in order to face the interview, competitive examination and various entrance test (CAT, GATE, GRE, MAT, Bank Exam, Railway Exam etc. A colony is defined as a visible mass of microorganisms all originating from a single mother cell. Play games, take quizzes, print and more with Easy Notecards. Classical biochemical tests are often used to identify microorganisms; the results are seen by color change. Bacteria can. biochemistry. Describe examples of biosynthesis products within a cell that can be detected to identify bacteria Accurate identification of bacterial isolates is essential in a clinical microbiology laboratory because the results often inform decisions about treatment that directly affect patient outcomes. Provide familiarity with procedures used for culturing (growing) and identifying microorganisms of medical importance. There is an online GLOSSARY OF TERMS, linked to terms within the text of the lab exercises. This is because of the lack of contrast coupled with the small size of the bacterial cell. UNKNOWN LAB REPORT. Unknown Number 116. Instructions. If unable to collect 3 mL of urine, collect in sterile specimen container (37777) or yellow top tube (104184) and transport urine specimens to the Microbiology Laboratory or refrigerate within 30 minutes. Todar's Online Textbook of Bacteriology presents information on Gram-positive aerobic endospore=forming bacteria, including members of the genus Bacillus. This does NOT mean that they are strict aerobes. tuberculosis or other acid-fast bacteria are present, though samples from elsewhere in the body (such as urine) may contain other Mycobacterium species. Areas covered: We searched in existing literature (Pubmed) for reports how MALDI-TOF MS has contributed to identification of rare and unknown bacteria from different groups. To identify unknown bacteria aseptic techniques were followed per the McDonald et al. Microbiology. Still, this document opens a new perspective of seeing the problem. Microbiology Discussion and Conclusion section of a microbiology lab report. GENERAL DISCUSSION. Describe the viruses. Each test performed, provided us with some key information about the unknown organism in question and how the bacteria. Uploaded by. | Gram Negative Unknown | Biology 3444-006 | | Lena Wallace | 11/7/2011 | | Abstract: The purpose of this lab was to identify an unknown bacteria culture using differential tests. We also offer a range of biochemical reagents, known for the identification of specific metabolisms and to differentiate between bacteria. In this paper I will discuss the processes of how I came to find my two unknown bacteria. al (1), unless noted within this lab report. A major innovation in laboratory sciences occurred in the late 1960s, when manual miniaturized identification systems were first introduced into the clinical microbiology laboratory. I had my times of mistakes trying to find out these bacteria from the rest but I stuck to the fundamental basics of the lab manual and believed in myself. What NOT to do A. In microbiology lab, you make three different culture plates: one general purpose plate, one selective media plate, and one differential media plate. Microbiology Laboratory Techniques Chapter Exam Take this practice test to check your existing knowledge of the course material. Animation. General Microbiology Concordia University, Irvine February 24, 2012 Identifying Salmonella choleraesuis using Several Microbiology Lab Techniques The purpose of this study was to determine what an unknown bacteria was using several different microbiology lab techniques including an API test, an oxidase test, a gram stain, a hanging drop. Unknown Bi 324 lab T / R 8pm Evaluation and Conclusion The unknown bacteria was grown in various media, observed, and tested. The American Society for Microbiology (ASM) is the oldest and largest single life science membership organization in the world. Bacteria grow on solid media as colonies. In later labs we will use a wide variety of special purpose differential media frequently used in the clinical laboratory to identify specific pathogenic. The laboratory manual procedures by McDonald et. Cultivation of Viruses. Greater confidence in. The biochemical tests in microbiology they are a set of chemical tests that are made to the microorganisms present in a sample in order to identify them; These microorganisms are usually bacteria. Tubes must be filled to 3 mL to prevent inhibition of bacterial growth. Unknown Number 109. Morphology and. MICROBIOLOGY MODULE Bacterial Identification Tests Microbiology 122 Notes 11 BACTERIAL IDENTIFICATION TESTS 11. The unknown bacteria will be one of the following: Enterococcus faecalis, Staphylococcus saprophyticus, Escherichia coli, Enterobacter aerogenes, Proteus vulgaris, Salmonella [I assume typhimurium], or Shigella [either flexneri or sonnei, we used both in our lab during the semester]. Describe examples of biosynthesis products within a cell that can be detected to identify bacteria; Accurate identification of bacterial isolates is essential in a clinical microbiology laboratory because the results often inform decisions about treatment that directly affect patient outcomes. Todar's Online Textbook of Bacteriology presents information on Gram-positive aerobic endospore=forming bacteria, including members of the genus Bacillus. ) with full. edu is a platform for academics to share research papers. Identification of Unknown Bacteria Identify unknown bacteria. Introduction Identification of unknown bacteria will allow the student to review. Understand the inoculations used throughout this laboratory exercise, use Table 4 as a study tool. (3 pts) Section 2 Biochemical Tests. unknown bacteria identification flow chart. 1 Identification of an Unknown Bacterium and Writing Up a Report A standard part of nearly all lab courses in introductory microbiology is an activity wherein the student must use everything that has been learned in the course to identify and unknown bacterial culture. Microbiology 210 Lab Final Unknown Report Section#006/ Radha. Lab #1 video clips Download Lab 1 ; Lab #2 Download Lab 2; First Unknown Dichotomous Keys for labs 2-4 (Printable Version. LAB REPORT Biochemical identification of unknown bacteria Unknown ID 001 Nerjes Alsalman 3/18/09 Biochemical identification of unknown bacteria RESULTS: Morphological characteristics: Nutrient Agar Slant: The tube in 37oC: The amount of growth was slight, white in color, and had effuse form of growth. Here is a sample of a section of the final lab report that must be handed in. A microorganism, or microbe, is a microscopic organism, which may exist in its single-celled form or in a colony of cells. When working in a microbiology lab, it is important to contain spills and discard materials properly. [ April 14, 2020 ] SARS-CoV-2 IgG/IgM Rapid Test for the diagnosis of COVID-19 Lab Diagnosis of Viral Disease [ April 4, 2020 ] Collection and Transport of Nasopharyngeal Swab Virology. Staining not only makes bacteria more easily seen, but it allows their morphology (e. Provide a flow chart to show different procedure involved in the identification process. The results of these tests on the suspected microorganism are then compared to known results for that organism to confirm its identification. Gram Negative Bacteria Kevin Le November 13, 2013 PURPOSE The focus of this study is to be able to identify an unknown bacterium, specifically a gram negative one,. Indeed, it has a slow rate of evolution (mutation) that make it perfect to build phylogenetic trees. April 29 th, 2014. What does the title "morphological" unknown tell you? Remember back to lab exercise 2-2, Cultural Characteristics. The Enterotube™ II is an example of a rapid, multi test system used in identification of unknown oxidase- negative, gram- negative, rod shaped bacteria of the family Enterobacteriaceae. In this simulation, you will learn to identify unknown bacteria. Out of a possible 70 points you will be graded as follows: Successfully performing the necessary tests to identify your unknown (25 pts):. coli from later generations would be more likely to give rise to cit+ cells. Isolation and Identification of Gram (+) Organism #13 - Bacillus subtilis 4. Learn microbiology lab exam 4 identification unknowns with free interactive flashcards. Urine isolates are tested by an automated system. coli because Mannitol have 9-11% NACL and every type of bacteria could not tolrate such amount of salt concentration. What were you studying in that lab?. Alcamo's Laboratory Fundamentals of Microbiology. The genus Pseudomonas contains more than 140 species, most of which are saprophytic. Information may be borrowed, but please site your sources correctly because of laws and regulations. Differential media contain dyes that react with the chemical processes of certain types of bacteria, allowing their identification. Differential staining methods, which typically require more than one stain and several steps, are referred to as such because they permit the differentiation of cell types or cell structures. Being able to culture, isolate and identify a unknown bacteria is one of the corner stones in microbiology BIO 203, as well as the ability to use different techniques to distinguish and identify different bacteria. Lab #1 video clips Download Lab 1 ; Lab #2 Download Lab 2; First Unknown Dichotomous Keys for labs 2-4 (Printable Version. Scientistcindy. 2012 - Unknown organism was streaked from a mixed broth onto both TSA and BHA plates in an attempt to isolate pure colonies for testing. Identification of Unknown Bacteria: · Differential Staining (Gram, Acid-fast, Endospore) · Interpret Unknown on MacConkey’s & Mannitol Salt Media · Identification of Unknown. Uploaded by. Microbiology’s art program enhances students’ understanding of concepts through clear and effective illustrations, diagrams, and photographs. General Microbiology Concordia University, Irvine February 24, 2012 Identifying Salmonella choleraesuis using Several Microbiology Lab Techniques The purpose of this study was to determine what an unknown bacteria was using several different microbiology lab techniques including an API test, an oxidase test, a gram stain, a hanging drop. You have reached a page from The Cleveland Clinic Disease Management Project that is no longer available on this web site. A schema diagram of how I identified the unknown is at the end of this report. Identification Matrix Screen. Microbiology 20 Biochemical Unknown - Spring 2009 (due May 14th) You should be prepared to turn in your notebook with your biochemical unknown identification completed after lab on Thursday May 14th. A colony is defined as a visible mass of microorganisms all originating from a single mother cell. The data were analyzed with respect to the use of economical and practical procedures for the accurate identification of lactose-fermenting and -nonfermenting Enterobacteriaceae within 24 hr after isolation. If an unknown species of bacteria is to be tested, divide a BAP plate in thirds. The oxidase test is used to identify bacteria that produce cytochrome c oxidase, an enzyme of the bacterial electron transport chain. All spills must be reported immediately. These microorganisms range from deadly to vital for life and health to continue. laboratory and are supervised by a scientist with general training in microbiology or a related science. Assignment. Identify each of the organisms worked with in this lab exercise. INTRODUCTION The identification of an unknown is beneficial in many ways, including the identification of bacteria in diseases, and in research. The greatest significance has been observed for bacteria that were challenging to be identified by traditional methods. Bacteria can. coli produces white colonies Blood agar is selective and differential media. Using algorithmic charts, the Virtual Lab mimics the linear process used for bacterial identification. TSA is tryptic soy agar. Catalase-positive Gram-positive bacilli, commonly called “diphtheroids” or “coryneform” bacteria were historically nearly always dismissed as contaminants when recovered from patients, but increasingly have been implicated as the cause of significant infections. A colony is defined as a visible mass of microorganisms all originating from a single mother cell. plate or tube inoculations on the first lab session in the week. Bacterial Unknown 2 The Virtual Microbiology Lab. Bacteria that ferment glucose in MR-VP media may produce large amounts of different kinds of acids (lactic, acetic, succinic, formic, etc. Expensive equipment and materials are not usually necessary for recovery of common aerobic or facultatively anaerobic bacterial pathogens, such as Staphylococcus spp, Streptococcus spp, and coliforms. ), carbon source utilization (Microlog; Biolog, Inc. In microbial ecology, the identification of microorganisms helps us characterize biodiversity. from a mixed culture This study was done in order to apply the various lab techniques used to identify bacterium that were covered in a. UNKNOWN LAB REPORT. I was given no clues as to what infectious inoculum was trapped within the confines of this test vial. 0 µm in diameter, usually seen in pairs with adjacent flattened sides (Figure 1 Left and Fig 2 below). In the identification of bacteria and fungi much weight is placed on how the organism grows in or on media. Understand the inoculations used throughout this laboratory exercise, use Table 4 as a study tool. Often used to differentiate species from the genera Clostridium and Bacillus. It is used differentiate gram negative rods particularly E. IDENTIFICATION OF UNKNOWN BACTERIA BIO 150 LAB. Using algorithmic charts, the Virtual Lab mimics the linear process used for bacterial identification. The use of stains that react chemically with cell material will. The following are a few examples of bacteria with unusual genomes. Identification of unknown bacteria using dichotomous key-Part 1 Medical Lab Lady Gill 15,810 views.